K2 Spice Drug Test – Synthetic Marijuana Urine Drug Testing

What are synthetic cannabinoids?
Synthetic Cannabinoids, a class of designer drugs, are available in head shops, tobacco stores, and over the Internet under a variety of names, such as, “K2”, “Spice”, “Tucatan Fire”, “Skunk”, “Moon Rocks”, “Black Mamba”, and “Bombay Blue”. They are often labeled “not for human consumption” and common routes of administration include inhalation and oral ingestion.1

Regular users of Synthetic Cannabinoids may experience withdrawal and addiction symptoms.2 Tolerance to these agents may develop rapidly, which might be associated with dependence.3 The compounds are stored in the body for a long period of time and long-term effects on humans are not known.1

In 2010, the American Association of Poison Control Centers received 2,906 calls related to synthetic cannabinoids. In 2011, the number of calls received increased to 6,959.4 On July 9, 2012, President Obama signed into law the Synthetic Drug Abuse Prevention Act banning synthetic compounds commonly found in synthetic marijuana, by placing them under Schedule I of the Controlled Substance Act.   5

Synthetic Marijuana and Drug Testing – Why Monitor?
Ameritox provides quantitative K2/Spice urine drug testing results for 13 of the most common synthetic cannabinoid compounds:

  • AM 2201 4-Hydroxypentyl
  • CP47, 497 C8 Hydroxy
  • JWH 018 5-Hydroxypentyl
  • JWH 018 Pentanoic Acid
  • JWH 073 4-Hydroxybutyl
  • JWH 073 Butanoic Acid
  • JWH 200 4-Hydroxylndole
  • JWH 200 6-HydroxyIndole
  • JWH 210 5-Carboxypentyl
  • JWH 210 5-Hydroxypentyl
  • RCS-4 5-Carboxypentyl
  • RCS-4 5-Hydroxypentyl
  • JWH 081 5-Hydroxypentyl
  • JWH 122 5-Hydroxypentyl
  • JWH 250 5-Hydroxypentyl

As of July 2012, these synthetic cannabinoids have been placed into Schedule 1 by the DEA, as “necessary to avoid imminent hazard to the public safety” due to high abuse potential and lack of medical use.1

Reported psychological effects of synthetic marijuana use2,6:

  • Paranoia
  • Panic attacks
  • Giddiness
  • Psychotic episodes
  • Hallucinations
  • Elevated mood
  • Relaxation
  • Altered perception

Reported physiologic of synthetic marijuana use2,6:

  • Tremor
  • Seizures
  • Nausea
  • Vomiting
  • Increased heart rate
  • Increased blood pressure
  • Mycardial ischemia
  • Heart attack

How do I request a test for synthetic cannabinoids?
Synthetic cannabinoids can be requested in Section 4 of a requisition form (the “Additional Tests” section). To add this test to a patient’s requisition, write in “K2”, “Spice” or “Synthetic Cannabinoids” in the “Other” section of Section 4. See images of Ameritox’s requisition form below to see where to write this in. Examples are shown for Ameritox’s current requisition form and older requisition form.

Section 4: Previous Requisition Form 

Section 4: Current Requisition

What do results look like in the Rx Guardian INSIGHT Report and Rx Guardian Results Report?
Positive results for Synthetic cannabinoids will appear on the Rx Guardian INSIGHT Report under “Illicit Drugs”. Below is an example of the Rx Guardian INSIGHT Report displaying a positive result for Synthetic Cannabinoids.

Positive results for synthetic cannabinoids will appear on the Rx Guardian Results Report under “Illicit Drugs” along with a Results Explanation. In the event of a positive result for Synthetic Cannabinoids, a Results Explanation will be present. Below is an example of the Rx Guardian Results Report displaying a positive result for Synthetic Cannabinoids.


1 US Department of Justice, Drug Enforcement Administration 2011 Drugs of Abuse Resource Guide.
2 National Institute of Health, National Institute of Drug Abuse (2012, May). DrugFacts: Spice (Synthetic Marijuana).
3 Vardakou, I., Pistos, C., & Spiliopoulou, C. (2010). Spice drug as a new trend mode of action, identification and legislation. Toxicology Letter, 197, 157-162.
4 American Association of Poison Control Centers, Synthetic Drugs Data and Fact Sheets, Bath Salts and Synthetic Marijuana (September 2012).
5 United States Department of Health and Human Services, Food and Drug Administration. Food and Drug Administration Safety and Innovation Act (FDASIA).
6 Spiller, HA, et. Al (2011). Clinical experience with and analytical confirmation of “bath salts” and “legal highs” (synthetic cathinones) in the United States. Clinical Toxicology. 49(6) 499-505.